Recombinant Proteinase 3 (PR3)说明书

Source

Prokaryotic expression

Host species

E.coli

Residues

Ala18~His246

Subcellular Location

Extracellular

Tags

N-terminal His Tag

Size

200μg/mL

Appearance

Freeze-dried powder

Purity

> 90%

Buffer formulation

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose

Predicted isoelectric point

7.0

Accurate Molecular Mass

26kDa as determined by SDS-PAGE reducing conditions

Predicted Molecular Mass

26.2KDa

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Storage instructions

Stable for 1 year at -80℃.

Aliquot to avoid repeated freezing and thawing.

Store at 2-8℃ for one month.

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence