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Recombinant Mineralocorticoid Receptor (MR)

发表时间:2023-10-20

Recombinant Mineralocorticoid Receptor (MR)

Catalog No : FY-P513515

Organism Species:Homo sapiens (Human)

Application:Positive Control; Immunogen; SDS-PAGE; WB.

 

 PROPERTIES

Source

Prokaryotic expression

Host species

E.coli

Residues

Val739~Lys984

Subcellular Location

Membrane, Nucleus, Cytoplasm, Endoplasmic reticulum lumen

Tags

N-terminal His and GST Tag

Appearance

Freeze-dried powder

Size

150ug/mL

Purity

> 90%

Buffer formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 0.01% SKL, 5%Trehalose

Predicted isoelectric point

6.6

Accurate Molecular Mass

59kDa as determined by SDS-PAGE reducing conditions. 

Predicted Molecular Mass

58.7KDa

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage instructions

Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence

VM

VLENIEPEIV

YAGYDSSKPD

TAENLLSTLN

RLAGKQMIQV

VKWAKVLPGF

KNLPLEDQIT

LIQYSWMCLS

SFALSWRSYK

HTNSQFLYFA

PDLVFNEEKM

HQSAMYELCQ

GMHQISLQFV

RLQLTFEEYT

IMKVLLLLST

IPKDGLKSQA

AFEEMRTNYI

KELRKMVTKC

PNNSGQSWQR

FYQLTKLLDS

MHDLVSDLLE

FCFYTFRESH

ALKVEFPAML

VEIISDQLPK

VESGNAKPLY

FHRK